Hiload 16 60 superdex 200 prep grade column ge healthcare. Store the column in 20% ethanol to prevent any microbial growth. Hiload 16600 superdex 200 prep grade are prepacked xk columns designed for highresolution preparative gel filtration chromatography. Hiload superdex 30, 75, and 200 pg columns bring convenience and high resolution to size exclusion chromatography. Sephadex g25 and g50, while superdex 75 prep grade and superdex 200 prep grade. Jan 01, 1996 polysaccharide leakage from superdex 30, 75 and 200 prep grade and sepharose hp in static experiments over a period of 4 weeks in different acidic conditions incubation superdex 30 superdex 75 superdex 200 sepharose hi solution prep grade prep grade prep grade mgml hci mgml mgml mgmo 0001 m 062 050 009 002 001 m 092 0. Superdex prep grade pg is a preparative gel filtration chromatography medium. Some characteristics of superdex gel filtration media are listed in appendix c, table 1. Is it possible to rescue, to some degree, a compressed gel filtration column superdex 75 or 200 1030 gl.
Superdex prep grade and prepacked hiload columns cytiva. Protein purification chromatography calibration of hiload. Superdex 75 prep grade a gives excellent resolution of the three proteins in the m r range 17 000 to 67 000 while the two largest elute together in the void volume. The purity is almost same, even very small proteins 20kda cant be separated from my. Jan 01, 1991 chromatography on superdex 200 prep grade at high and low sample volumes. Produce separation medium with high selectivity and resolution.
Superdex 200 prep grade pg are prepacked xk columns designed for preparative size exclusion. Superdex 200 prep grade gel filtration medium offers very high resolution fractionation for separation, polishing and product formulation of monoclonal antibodies. Hiload 16600 superdex 200 pg cytiva, 28989335, column l. Performance of superdex 200 prep grade measured as kav values of a protein mixture after repeated treatment with a 0.
The media also withstand the rigorous conditions used in process hygiene procedures such as sanitization. Maintenance and cleaning of size exclusion chromatography columns. Stability superdex pg can be used in aqueous solutions with a ph range of 3 to12 for continuous operation, and ph 1 to 14 for cleaninginplace cip. Superdex 200 prep grade cytiva, formerly ge healthcare life. Pressure vs flow velocity for superdex 200 prep grade in bpg 200 at 82. Superdex prep grade is a composite matrix of dextran and crosslinked agarose. Hiload superdex 200120ml or superdex 200 increase 24ml.
The upper exclusion limit for globular proteins that this column can handle is approximately 1. Superdex 200 prep grade pg are prepacked xk columns designed for. Ge healthcare superdex 200 increase 10300 gl superdex 200 increase 10300 gl is a versatile, prepacked column for size exclusion chromatography in smallscale mg preparative purification as well as for characterization and analysis of proteins with molecular weights between 10 000 and 600 000, such as antibodies. The concentrated sample was then loaded on to a calibrated hiload 1660 superdex 200 prep grade ge healthcare sizeexclusion column equilibrated with buffer a 50 mm trishcl, ph 7. Superdex 200 prep grade cytiva, formerly ge healthcare life sciences superdex 200 prep grade superdex 200 prep grade size exclusion chromatography resin offers very high resolution fractionation for separation, polishing and product formulation of monoclonal antibodies. The separation properties of these media are determined by the dextran componentchromatography columns separate molecules within mixtures during gas or liquid chromatography.
Influence of sample volume on resolution on superdex 200 prep grade at three different flowrates p 15. Cytivahiload 26600 superdex 200 pg hiload 2660 superdex. Superdex 30 prep grade, superdex 75 prep grade and superdex 200 prep grade may be used in aqueous solutions over the range ph 312 for continuous operation and over the range ph 114 for cip. Superdex 200 prep grade 20% ethanol at 4c to 30c 1 h 2 o at room temperature figure 6 illustrates the stability of superdex 200 prep grade in 0. Ge life sciences 28989335 hiload 16600 superdex 200 prep grade, prepacked column, 120ml currently unavailable. Superdex 75 prep grade gives excellent resolution for biomolecules from m r 3000 to 70 000 while superdex 200 prep grade allows fractionation in the m r 10 000 to 600 000 range. Ge life sciences 28989335 hiload 16600 superdex 200 prep. Superdex 75 prep grade and superdex 200 prep grade are ideal for all stages. Hiload 1660 or 2660 superdeprep grade hiload 1660 or 2660 superdex 75 prep grade hiload 1660 or 2660 superdex 200 prep grade dismantling tool, support screens, net rings.
Elution volumes for globular proteins of various molecular weights on superdex 75 increase 10300 blue, superdex 200 increase 10300 green, and superose 6 increase 10300 orange. Steep selectivity curves give unmatched resolution for biomolecules in the molecular weight range up to 10 000 for superdex 30 pg, 3 000 to 70 000 for superdex 75 pg, and 10 000 to 600 000. Steep selectivity curves give excellent resolving power for proteins and other biomolecules in the molecular weight range. Steep selectivity curves give unmatched resolution for biomolecules in the molecular weight range up to 10 000 for superdex 30 pg, 3000 to 70 000 for superdex 75 pg, and 10 000. Superdex 200 prep grade b resolves these two largest proteins. Preparation for downstream analyses principles and methods 28962400 ge healthcar e life sciences pr otein sample pr eparation handbook protein sample preparation handbook 28988741 ge healthcar e life sciences purifying challenging pr oteins principles and methods purifying challenging proteins principles and methods 28909531 ge healthcar e. Superdex 200 10300 gl chromatographic separation column from. The superdex 200 10300 gl column has a mean bead size um and the fractionation range for the globular proteins is 10,000 600,000.
It may be easier to buy the 34um bead size prep grade matrix and. Hiload gel filtration columns from pharmacia university of guelph. Hiload superdex 75 prep grade data file hiload superdex 200. These instructions contain information about superdex pg. Hiload superdex 200 pg preparative sec columns cytiva. This sec resin combines excellent mechanical strength with high hydrophilicity, allowing high flow rates and minimal nonspecific interactions. Size exclusion chromatography superdex prep grade and. Comparison of the selectivity of superdex 75 prep grade and superdex 200 prep grade for model proteins.
Superdex 200 preparative grade gel filtration column, supplied by ge healthcare, used in various techniques. Hiload superdex 200 preparation grade resin is well suited for rapid. Rak design ab principles and methods gel filtration. Superdex 200 prep grade pg are prepacked xk columns designed for preparative gel filtration.
Procedure 29140760 aa chromatography systems maintenance. The steep selectivity of dextran and the high chemical and physical stability of agarose enable high resolution separations. General description hiload 16600 superdex 200 prep grade are prepacked x k columns designed for highresolution preparative gel filtration chromatography. Hiload superdex 30, 75, and 200 prep grade columns bring. Superdex prep grade superdex prep grade pg is a preparative gel filtration chromatography. Hiload 16600 and 26600 superdex 30 prep grade, hiload 16600 and 26600 superdex 75 prep grade, and hiload 16600 and 26600 superdex 200 prep grade pg are prepacked xk columns designed for preparative gel filtration. Filled with stationary phase compositions or chemical compounds, the prepacked options reduce the preparing time required. Ge healthcare size exclusion chromatography superdex 75. With their high physical and chemical stability, very high batchtobatch reproducibility, and regulatory support file backup, superdex 30 prep grade, superdex 75 prep grade and superdex 200 prep grade are ideal for all stages of an industrial scale operation from research and. This matrix combines the excellent gel filtration properties of dextran with the physical and chemical stability of highly crosslinked agarose. Dextran component determined the separation properties of these media.
Characteristics of superdex prep grade media for gel. We dont know when or if this item will be back in stock. Connect the transport tool to the column outlet, to prevent air entering the column. Hiload superdex 200 prepgrade chromatography buffer solution.
This configuration has an automatic, selfcleaning injection valve for sample introduction, and fullspectrum uv 200 400 nm or uvvis 200 800 nm detection with secondary wavelength. Instructions 56119099 ae gel filtration media superdex 30. Rapid purity check and screening superdex 75 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. The column is an excellent choice for separating mabs from dimers and contaminants.
Exceptional resolution with short run times and good recovery. To prevent any nonspecific interactions with the resins, it is recommended to use an eluent with an ionic. Superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose. This column is packed with superdex 75 prep grade pg resin.
Jan 12, 2010 the gel filtration properties of the superdex matrix are governed mainly by the dextran chains. Superdex prep grade is a composite matr ix of dextran and highly crosslinked agarose. Ge healthcare superdex 200 increase 10300 gl for sale labx. Pressure flow velocity curves in bpg 200 with a bed height of 60 cm. Superdex 200 preparative grade gel filtration column ge.
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